Description
Alker
Alkaline Lysis System
The Alker system performs controlled extraction of plasmid DNA (pDNA) from E. Coli following a continuous alkaline lysis manufacturing process. The reactant ratios are monitored and controlled in real-time by a feedback control algorithm, adding to the robustness provided by theoretical calculations. Lysis time is controlled automatically by adjusting pump speeds based on feedback from the digital scales. The system provides advanced process analytics and precise process parameter controls, making it perfectly suited for pDNA/mRNA process development and characterization activities. The system is suitable for manufacturing processes in either the 0.1L ~ 30L or 20L ~ 200L scales, and a 21CFR11 compliant software package is available as an add-on for GMP settings.
System Summary
- Real-time control of lysis time and alkaline/neutralization ratios
- Intuitive minimalist GUI and simple method editor
- Fully disposable flow-path and easily movable modular design
- Automated pump tube priming and air venting
Key Features
– Fully disposable flow path
– Designed for upstream processes of up to 3L
– Dead volumes < 50 mL
– Scales monitor solution masses in real-time up to 5kg (at 0.1g increments)
– Peristaltic pumps provide low-shear flows from 20 – 2300 ml/min
– Liquid sensors capable of non-invasive detection across thicknesses of up to 10 mm
– Compatible with a wide range of in-line axial mixers
– Runs in both manual and fully-automatic modes
– Real-time mass flow feedback ensures simultaneous completion of continuous steps
– Data export of raw data in XLSX
Key Specifications
| Manufacturing Scale | Digital Scale |
| 0.1L ~ 30L | max. 30kg (±0.1g) |
| 20L ~ 200L | max. 60kg (±1g) or
max. 200kg (±5g) |
| Pump Head | Size | ID (mm) | Flowrate (L/min) |
| Standard | #40 | 4.0 | 0.8 |
| Standard | #25 | 4.8 | 1.0 |
| Standard | #17 | 6.4 | 1.7 |
| Standard | #18 | 7.9 | 2.4 |
| High Performance | #15 | 4.8 | 2.0 |
| High Performance | #24 | 6.4 | 3.0 |
| High Performance | #35 | 7.9 | 5.0 |
| High Performance | #36 | 9.6 | 6.0 |
System Specifications
| Size | 200 x 75 x 150 cm |
| Weight | <20 kg |
| Software | Alker control software |
| Networking | Ethernet (or WiFi) |
| Plug | 100 – 240 VAC, 50/60 Hz |
| Power consumption | 470W |
| Maximum flowrates | 0.8 – 6.0 L/min |
| Minimum pump speed | 0.1 rpm |
| Maximum scale weights | 30 – 200 kg |
| Liquid detection sensor | Capacitance |
| Operating conditions | 4 – 35 °C, 20 – 80% humidity |
| Noise | < 66dB |
Ordering Information
Parts list
| Alker control software | 10000 |
| 24 CFR part 11 software add-on | 10001 |
| Tablet computer | 10002 |
| Control box | 10100 |
| Pump aviation connector cable | 10110 |
| Pump, standard head | 10111 |
| Pump, high performance head | 10112 |
| Digital scale cable | 10120 |
| Digital scale, 30kg | 10121 |
| Digital scale, 60kg | 10122 |
| Digital scale, 200kg | 10123 |
| Liquid detection sensor | 10130 |
| Disposable solution bag | 10140 |
| Disposable solution bottle with inserts | 10141 |
| Disposable flow-path tubing set | 10142 |
| Disposable tubing connector kit | 10143 |
| Stand, optimized for air venting/purging | 10150 |
| Static mixer module, stainless steel | 10160 |
| Static mixer module, disposable | 10161 |
| Lysis loop stand | 10170 |
Contact Information
Molsil Scientific
Union City, CA
Website: www.molsil.com
Email: mol-sil@outlook.com
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