Pump calibration using Alker software
Pump calibration is necessary to translate flow rate units of milliliters per minute to revolutions per minute (RPM) signals used by the controller box to communicate with the pumps. A linear approximation is used where the y-intercept is set to 0 and the slope is set to the calibration value measured in milliliters per revolution.…
Overcoming viscosity variability during alkaline mixing
A well-known problem with the lysis operation is the real-time increase in viscosity when the NaOH-SDS buffer mixes with resuspended bacterial cells. This results in pumps needing to be adjusted during the process in real-time. Changes in viscosity are mainly observed in the alkaline step and are caused by degraded genomic DNA passing through narrow…
gmp production
Crosser TFF
• Personalized manufacturing ready
• High dynamic range: 2mL to 500L UF/DF
• Small factory footprint: 45 cm2
• Pressure control: ±0.05 psi, latency: 2 min
• GMP ready, clinically proven
• 100% single-use flow path
atmp process development
Alker 3L
The only off-the-shelf bioprocessing solution for alkaline lysis of plasmid DNA at 0.1 – 3L fermentation scales. Validated for pDNA process development in fast-paced clinical manufacturing projects. Web-based software provides flexible automation, PID-controlled process parameters, and minimal operator training. Fully computer-controlled processes at small scales facilitate future scale-up studies.
